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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: Efficient generation of GGTA1-null Diannan miniature pigs using TALENs combined with somatic cell nuclear transfer

Fig. 2

TALEN-mediated GGTA1 mutations in PFFs. a PCR product from the TALEN target locus in GGTA1-modified cell lines. b Detection of the GGTA1 gene in cell colonies by PCR. The genomic regions surrounding the target site were amplified and a 752-base-pair PCR product of the GGTA1 gene was obtained. c Genotyping of GGTA1-mutant cell lines by the T7EI assay. The GGTA1 gene of each cell colony was assayed and presented in the same order as the PCR results. Individuals with one band of the wild-type (WT) and mutated alleles show three bands in the T7EI assay. d Representative sequencing chromatographs of the complementary sequence to the TALEN target site in C99# GTKO cell line

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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