The participation of the muscarinic receptors in the preoptic-anterior hypothalamic areas in the regulation of ovulation depends on the ovary

Table 5 Effects of ULO performed at 13:00 h on diestrus-2 day on hormone serum levels at 11:00 or 17:00 h on predicted proestrus day

Group	Estradiol pg/mL	LH ng/mL	FSH ng/mL
Proestrus 11:00 h
Intact	20.3 ± 0.3	0.7 ± 0.30	2.3 ± 0.4
Left laparotomy	14.5 ± 4.0	0.4 ± 0.06	2.0 ± 0.1
Left ULO	61.4 ± 16.1^ab	1.3 ± 0.20^a	42.1 ± 14.7^ab
Right laparotomy	13.0 ± 4.8	0.4 ± 0.01	2.7 ± 0.6
Right ULO	12.4 ± 3.5	2.2 ± 0.40^a	>100^a
Proestrus 17:00 h
Intact	15.7 ± 2.9	22.8 ± 1.90	7.2 ± 0.50
Left laparotomy	10.8 ± 0.5	12.5 ± 0.01	5.2 ± 0.50
Left ULO	11.5 ± 2.1	0.5 ± 0.10^a	<0.040^ab
Right laparotomy	10.7 ± 1.3	19.4 ± 0.2	8.8 ± 0.8
Right ULO	15.9 ± 3	1.4 ± 0.10^a	2.1 ± 1.04^a

The mean ± SEMs for the estradiol, LH and FSH of rats subjected to left or right laparotomy, and left or right ULO at 13:00 h of diestrus-2 day, and sacrificed at 11:00 or 17:00 h on the predicted proestrus day
^a p < 0.01 vs. corresponding laparotomy (MANOVA, followed Tukey test)
^b p < 0.02 vs. Right ULO (Student “t” test)

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