The participation of the muscarinic receptors in the preoptic-anterior hypothalamic areas in the regulation of ovulation depends on the ovary

Table 4 Effects of atropine microinjected in the left or right POA-AHA on hormone serum levels at 11:00 or 17:00 h on predicted proestrus day

Group POA-AHA	Estradiol pg/mL	LH ng/mL	FSH ng/mL
Proestrus 11:00 h
Intact	20.3 ± 0.3	0.7 ± 0.3	2.3 ± 0.4
Left vehicle	32.4 ± 9.0	0.4 ± 0.05	1.6 ± 0.13
Left atropine	27.2 ± 5.5	0.2 ± 0.03	2.4 ± 0.8
Right vehicle	33.4 ± 4.9	0.5 ± 0.02	1.2 ± 0.09
Right atropine	21.6 ± 1.9	0.4 ± 0.09	1.2 ± 0.3
Proestrus 17:00 h
Intact	15.7 ± 2.9	22.8 ± 1.9	7.2 ± 0.50
Left vehicle	23.6 ± 6.0	24.9 ± 2.5	4.9 ± 2.5
Left atropine	3.9 ± 0.9^abc	3.6 ± 1.5^a	98.8 ± 4.4^abc
Right vehicle	37.2 ± 1.3	20.7 ± 1.8	4.1 ± 0.2
Right atropine	33.2 ± 10.2	21.2 ± 2.6	8.3 ± 2.5

The mean ± SEMs for the estradiol, LH and FSH of rats subjected to microinjection of vehicle or atropine into the left or right POA-AHA, at 13:00 h of diestrus-2 day, and sacrificed at 11:00 or 17:00 h on the predicted proestrus day
^a p < 0.01 vs. Left vehicle (ANOVA, followed Tukey test)
^b p < 0.01 vs. right atropine (ANOVA, followed Tukey test)
^c p < 0.01 vs. intact group (ANOVA, followed Tukey test)

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