The participation of the muscarinic receptors in the preoptic-anterior hypothalamic areas in the regulation of ovulation depends on the ovary

Table 1 Ovulation in rats with unilateral atropine microinjection into POA-AHA performed at 13:00 h of diestrus-2 day

Group	Side of POA-AHA	n	Ovulation rate*	Ova shed
Group	Side of POA-AHA	n	Ovulation rate*	Left ovary	Right ovary
Intact	–	10	10/10	7.1 ± 0.5	5.4 ± 0.3
Vehicle	Left	9	9/9	6.1 ± 0.9	4.2 ± 1.0
Vehicle	Right	9	9/9	6.5 ± 0.2	4.8 ± 0.3
Atropine	Left	10	2/10^a	4, 0**	3, 3**
Atropine	Right	10	10/10	3.1 ± 0.3^b	1.7 ± 0.2^b

The ovulation rate and the number of ova shed (mean ± SEMs) by rats subjected to microinjection of the vehicle or atropine into the left or right side of the POA-AHA region were measured at the predicted estrus day
^a p < 0.0007 vs. left vehicle (Fisher’s exact probability test)
^b p < 0.001 vs. right vehicle (Kruskall Wallis test, followed Dunn test)
**represents the number of ova shed by the two ovulating rats
*Number of ovulating animals/number of treated animals

ISSN: 1477-7827