Fig. 4
From: Characterization of exosomal release in bovine endometrial intercaruncular stromal cells
Characterization of exosomes release from 8 % O2 and 1 % O2 ICAR cell-conditioned media. Exosomes were characterized after enrichment from the 100,000 x g pellet by buoyant density centrifugation (see Methods). a Representative Western blot for exosome markers: TSG101 and CD63. b Representative electron micrograph exosome fractions, Scale bar 100 nm. c Representative Nanosight measurement of particle-size distribution exosomes from 8 % O2 and 1 % O2 cell-conditioned media after buoyant density gradient ultracentrifugation. (8 % normoxic condition mean size (127 ± 1.7 nm) (○), 1 % hypoxic condition mean size (123 ± 2.7 nm) (●) over a period of 48 h). d Exosomes concentration presented as vesicle per million cells per 48 h was higher (p < 0.05) at hypoxia (1 % O2) compared to normal oxygen tension (8 % O2); 8 % O2 (white bar) and 1 % O2 (black bar). Data are presented as mean ± SE, n = 3