Fig. 3
From: Characterization of exosomal release in bovine endometrial intercaruncular stromal cells
The effects of different oxygen tension on activation of apoptotic protein caspase-3 of bovine endometrial stromal cells (ICAR). ICAR cells were cultured under normoxic (8 % O2) or hypoxic (1 % O2) conditions and the activated caspase-3/7 fluorescence was measured at 48 h. A Representative phase-contrast images (a and d), fluorescent signal images (b and e) and acquired fluorescent signal using integrated object counting algorithm with IncuCyte™ (Segmentation; c and f), Scale bar 400 μm. B Increased apoptosis of ICAR cells under hypoxic conditions as determined by acquired fluorescent signal using integrated object counting algorithm with IncuCyte™ were normalized against cell confluence, 8 % O2 (white bar) and 1 % O2 (black bar). Data are presented as mean ± SE, n = 6. In (B) P < 0.05