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Table 1 Efficacy of CRISPR/Cas9 system to induce targeted disruption of IL2RG during embryogenesis in vitro. CRISPR/Cas9 system at the concentration of 10 ng/ul each sgRNAs and 20 ng/ul Cas9 mRNA was introduced into pig zygotes. Genotyping of subsequent embryos on day 7 demonstrated that all embryos carried mutation on IL2RG; no wild type sequence was found from genotyping

From: Biallelic modification of IL2RG leads to severe combined immunodeficiency in pigs

Concentration of CRISPR/Cas9 (ng/uL) # of embryos injected % of blastocyst on day 7 (number of blastocysts/cleaved) # of blastocyst genotyped Genotypes
Homozygous Mutation Biallelic Mutation Mosaic Mutation Heterozygous Mutation Wild-type
10/20 70 25.71 %
4 0 2 2 0 0