Skip to main content
Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: Biallelic modification of IL2RG leads to severe combined immunodeficiency in pigs

Fig. 1

Use of CRISPR/Cas9 system to disrupt IL2RG in pigs. a Design of CRISPRs to target IL2RG. Sequences in green and blue indicate target sequences and letters in red reflect PAM (NGG) sequences. Red arrows indicate the location of primers used to genotype embryos and piglets. Black arrows indicate the location of extended primers. b Schematic strategy used to produce IL2RG deficient pigs. IL2RG knockout cells lines were established from fetuses derived from CRISPR/Cas9 injected embryos. SCNT was performed to generate IL2RG knockout female pigs. c PCR amplification to genotype IL2RG knockout fetuses. Various mutations were detected from fetus 1, 2, 4, and 5. d Genotype of IL2RG knockout cell line used for SCNT 4. Bold letters indicate insertion or change in nucleotides, and ‘-’ indicates deletion of nucleotide

Back to article page