Table 1 Primers list used in RT-qPCR
From: Modulation of gonadotrophin induced steroidogenic enzymes in granulosa cells by d-chiroinositol
Gene | Protein name | Sequence 5′- 3′ | Amplicon lenght (bp) | NCBI Ref. sequence |
|---|---|---|---|---|
RpS7 | Ribosomal protein S7 | F: AATCTTTGTTCCCGTTCCTCA | 135 | NM_001011.3 |
R: CGAGTTGGCTTAGGCAGAA | ||||
β3 integrin | β3 integrin | F: GACAAGGGCTCTGGAGACAG | 233 | NM_000212.2 |
R: ACTGGTGAGCTTTCGCATCT | ||||
IGF1R | Insulin-like growth factor 1 receptor | F: CGTGGGAGGGTTGGTGATTA | 161 | NM_000875.3 |
R: TGGCCACTCTGGTTTCAGGT | ||||
CYP19A1 | Aromatase | F: CCCTTCTGCGTCGTGTCAT | 86 | NM_000103.3 |
R: GATTTTAACCACGATAGCACTTTCG | ||||
P450scc (CYP11A1) | Cholesterol side-chain cleavage enzyme | F: ACCAAGAACTTTTTGCCCCT | 127 | NM_000781.2 |
R: ATGTCCCCCGAGTAATTTCC |