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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: 17β-estradiol modifies human spermatozoa mitochondrial function in vitro

Fig. 3

Changes of percentage of spermatozoa with high and low mitochondrial membrane potential after E2 stimulation. R2 and R3 gates represent cells with high (JC-1 aggregates emitting red to orange fluorescence) and low (JC-1 monomers emitting green fluorescence) mitochondrial membrane potential after E2 stimulation, respectively. Representative reactions: a-c increase of percentage of high ΔΨ spermatozoa after 10−8 M E2 stimulation; eg-decrease of percentage of high ΔΨ spermatozoa after 10−6 M E2 stimulation. d, h-controls after 120 min stimulation

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