Fig. 1
From: 17β-estradiol modifies human spermatozoa mitochondrial function in vitro
Kinetics of intracellular free calcium ions concentration changes in human spermatozoa after E2 stimulation. a Spermatozoa stained with Fluo-3 reveal high concentration of free calcium ions in midpiece. b Representative reaction after stimulation with 17β-estradiol in 10−10 M, 10−8 M or 10−6 M final concentration. The individual lines in the graphs correspond to the reactions of individual sperm cells. Arrows indicate the moment of E2 administration. Ch1-fluorescence channel for Fluo-3