Fig. 1

Sanger sequencing chromatograms of mutated loci in patients and their family members. a Sequencing chromatogram of the exon 1 of GNRH1 gene in Patient 1 and Patient 2 genomes. b Sequencing chromatogram of the exon 1 of GNRHR gene in Patient 1 and Patient 2 genomes. c Sequencing chromatogram of the exon 3 of GNRHR gene in Patient 1 genomes. d Sequencing chromatogram of the exon 1 of GNRHR gene in the genomes of mothers of Patient 1 and Patient 2. e Sequencing chromatogram of the exon 1 of GNRHR gene in the genome of Patient 1 offspring