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Table 2 Primer pairs for amplification of reference genes

From: mRNA expression pattern of selected candidate genes differs in bovine oviductal epithelial cells in vitro compared with the in vivo state and during cell culture passages

Gene Sequence of nucleotide Accession no. /Reference Product size (bp) Tm (°C)
HDAC1 for 5′ –CCA GTG CAG TTG TCT TGC AG- 3′ NM_001037444.2 217 60
rev 5′ –TTA GGG ATC TCC GTG TCC AG- 3′
UXT for 5′ –CGC TAC GAG GCT TTC ATC TC- 3′ NM_001037471.2 207 61
rev 5′ –TGA AGT GTC TGG GAC CAC TG- 3′
PPIA for 5′ – CTG AGC ACT GGA GAG AAA GG- 3′ NM_178320.2 259 60
rev 5′ – TGC CAT CCA ACC ACT CAG TC- 3′
RPL19 for 5′ -GGC AGG CAT ATG GGT ATA GG- 3′ NM_001040516.1 232 60
rev 5′ -CCT TGT CTG CCT TCA GCT TG- 3′
SUZ12 for 5′ -TTC GTT GGA CAG GAG AGA CC- 3′ [59] 286 60
rev 5′ -GTG CAC CAA GGG CAA TGT AG- 3′
  1. Selected gene transcripts, primer sequences and annealing temperatures (Tm) used for normalization of quantitative PCR with resulting amplicon length