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Table 1 Primer pairs for amplification of genes of interest

From: mRNA expression pattern of selected candidate genes differs in bovine oviductal epithelial cells in vitro compared with the in vivo state and during cell culture passages

Gene Sequence of nucleotide Accession no. /Reference Product size (bp) Tm (°C)
PTGS1 for 5′ -CAG ATG CGG AGT TTC TGA GTC G- 3′ [27] 313 60
rev 5′ -GGG TAG TGC ATC AGC ACG G- 3′
PTGS2 for 5′ -CTC TTC CTC CTG TGC CTG AT- 3′ [27] 359 60
rev 5′ -CTG AGT ATC TTT GAC TGT GGG AG- 3′
PTGES2 for 5′ -CCT CCT ACA GAA AGG TGC C- 3′ [56] 133 56
rev 5′ -GTG ATG ATG TCT GCC AGG G- 3′
PTGES3 for 5′ -TGC AAA GTG GTA CGA TCG G- 3′ [56] 253 61
rev 5′ -TAA CCT TGG CCA TGA CTG G- 3′
MUC1 for 5′ -ATG ACC ACC CGC TCT ATG TC- 3′ AJ400824 189 60
rev 5′ -GGA GGT GGA AAG TGC TAT GC- 3′
MUC4 for 5′ -ACG TCA CTG TGC ATC TTT GG- 3′ XM_002684819.3 199 60
rev 5′ -AAG CTC TTG ATG GAC GGT TG- 3′
MUC6 for 5′ -CAG CAG TCC CAC TTC CTC TG- 3′ XM_005197398.3 206 65
rev 5′ -CAG TGA TGG AGC TGG CTA GG- 3′
MUC16 for 5′ -CAG GTC TCA AAA TCC CAT CC- 3′ XM_002688785.3 256 62
rev 5′ -TGC TGG AGG TGT TGA TAT GG- 3′
OVGP1 for 5′ -GGG AAA GGT TCG TCA GTT CA- 3′ NM_001080216.1 240 60
rev 5′ -CAT ACG CTT TCT GGA CGA CA- 3′
BDH2 for 5′ -ATG TCC TCT GTG GCT TCC AG- 3′ NM_001034488.2 347 59
rev 5′ -CAC AAA CTC CAG CCT CCA TC- 3′
OXCT2 for 5′ -CAC AGT GAG AAC GGG ATC TTG- 3′ XM_002704022.2 347 55
rev 5′ -GTG CAC TTC TCC ACG ATC TTG- 3′
GAPDH for 5′ -CCC AGA AGA CTG TGG ATG G- 3′ [57] 306 62
rev 5′ -AGT CGC AGG AGA CAA CCT G- 3′
SDHA for 5′ -GGG AGG ACT TCA AGG AGA GG- 3′ [57] 219 60
rev 5′ -CTC CTC AGT AGG AGC GGA TG- 3′
HK1 for 5′ -GCG TTT CCA CAA GAC TCT GC- 3′ XM_010820525 324 61
rev 5′ -AGA TCC AGG GCC AAG AAG TC- 3′
IL8 for 5′ -CGA TGC CAA TGC ATA AAA AC- 3′ [58] 153 56
rev 5′ -CTT TTC CTT GGG GTT TAG GC- 3′
  1. Selected gene transcripts, primer sequences and annealing temperatures (Tm) used for quantitative PCR with resulting amplicon length