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Table 1 Primer pairs for amplification of genes of interest

From: mRNA expression pattern of selected candidate genes differs in bovine oviductal epithelial cells in vitro compared with the in vivo state and during cell culture passages

Gene

Sequence of nucleotide

Accession no. /Reference

Product size (bp)

Tm (°C)

PTGS1

for 5′ -CAG ATG CGG AGT TTC TGA GTC G- 3′

[27]

313

60

rev 5′ -GGG TAG TGC ATC AGC ACG G- 3′

PTGS2

for 5′ -CTC TTC CTC CTG TGC CTG AT- 3′

[27]

359

60

rev 5′ -CTG AGT ATC TTT GAC TGT GGG AG- 3′

PTGES2

for 5′ -CCT CCT ACA GAA AGG TGC C- 3′

[56]

133

56

rev 5′ -GTG ATG ATG TCT GCC AGG G- 3′

PTGES3

for 5′ -TGC AAA GTG GTA CGA TCG G- 3′

[56]

253

61

rev 5′ -TAA CCT TGG CCA TGA CTG G- 3′

MUC1

for 5′ -ATG ACC ACC CGC TCT ATG TC- 3′

AJ400824

189

60

rev 5′ -GGA GGT GGA AAG TGC TAT GC- 3′

MUC4

for 5′ -ACG TCA CTG TGC ATC TTT GG- 3′

XM_002684819.3

199

60

rev 5′ -AAG CTC TTG ATG GAC GGT TG- 3′

MUC6

for 5′ -CAG CAG TCC CAC TTC CTC TG- 3′

XM_005197398.3

206

65

rev 5′ -CAG TGA TGG AGC TGG CTA GG- 3′

MUC16

for 5′ -CAG GTC TCA AAA TCC CAT CC- 3′

XM_002688785.3

256

62

rev 5′ -TGC TGG AGG TGT TGA TAT GG- 3′

OVGP1

for 5′ -GGG AAA GGT TCG TCA GTT CA- 3′

NM_001080216.1

240

60

rev 5′ -CAT ACG CTT TCT GGA CGA CA- 3′

BDH2

for 5′ -ATG TCC TCT GTG GCT TCC AG- 3′

NM_001034488.2

347

59

rev 5′ -CAC AAA CTC CAG CCT CCA TC- 3′

OXCT2

for 5′ -CAC AGT GAG AAC GGG ATC TTG- 3′

XM_002704022.2

347

55

rev 5′ -GTG CAC TTC TCC ACG ATC TTG- 3′

GAPDH

for 5′ -CCC AGA AGA CTG TGG ATG G- 3′

[57]

306

62

rev 5′ -AGT CGC AGG AGA CAA CCT G- 3′

SDHA

for 5′ -GGG AGG ACT TCA AGG AGA GG- 3′

[57]

219

60

rev 5′ -CTC CTC AGT AGG AGC GGA TG- 3′

HK1

for 5′ -GCG TTT CCA CAA GAC TCT GC- 3′

XM_010820525

324

61

rev 5′ -AGA TCC AGG GCC AAG AAG TC- 3′

IL8

for 5′ -CGA TGC CAA TGC ATA AAA AC- 3′

[58]

153

56

rev 5′ -CTT TTC CTT GGG GTT TAG GC- 3′

  1. Selected gene transcripts, primer sequences and annealing temperatures (Tm) used for quantitative PCR with resulting amplicon length