Divergent activity of the gonadotropin-releasing hormone receptor gene promoter among genetic lines of pigs is partially conferred by nuclear factor (NF)-kB, specificity protein (SP)1-like and GATA-4 binding sites

Table 1 Sense strand of EMSA oligos^a

Name	Sequence
AP2 consensus^b	5’-GATCGAACTGACCGCCCGCGGCCCGT-3’
GATA consensus^b	5’-CACTTGATAACAGAAAGTGATAACTCT-3’
GR consensus^b	5’-AGAGGATCTGTACAGGATGTTCTAGAT-3’
NF1 consensus^b	5’-TTTTGGATTGAAGCCAATATGATA-3’
NF-κB consensus^b	5’-AGTTGAGGGGACTTTCCCAGGC-3’
SP1 consensus^b	5’-AATCGATCGGGGCGGGGCGAG-3’
C/I −855/−835^c	5’-GCATACAAAGGGATATAAACA-3’
M −853/−833^c	5’-GCATACAAAGTGATATAAAC-3’
C/I −1245/−1225^c	5’-AGCTTCCTCACGGCCTGGATG-3’
M −1243/−1223^c	5’-AGCTTCCTCAGGGCCTGGATG-3’
C/I −1700/−1680^c	5’-AACCCCATATTTCCACTGAGA-3’
M −1676/−1656^c	5’-AACCCCATATCTAGGCACTAA-3’

^aThe complement strand was annealed for each oligonucleotide prior to use in gel shift assays
^bAP2, activator protein 2; GR, glucocorticoid receptor; NF1, nuclear factor 1; NF-B, nuclear factor-κB; SP1, specificity protein 1
^cBase pair substitutions between the different breed promoters are in bold italics

ISSN: 1477-7827