Skip to main content
Fig. 6 | Reproductive Biology and Endocrinology

Fig. 6

From: Divergent activity of the gonadotropin-releasing hormone receptor gene promoter among genetic lines of pigs is partially conferred by nuclear factor (NF)-kB, specificity protein (SP)1-like and GATA-4 binding sites

Fig. 6

Mutation of GATA-4 and NF-κB elements indicate those sites are functionally relevant to promoter activity. To confirm the importance of the GATA-4 binding site within the Meishan promoter and the NF-κB element within the Control/Index promoters, block replacement mutations of each binding site were constructed within the context of their respective full length promoter. Transient transfection of αT3-1 cells with luciferase (LUC) reporter vectors containing both full-length promoters (−M5118pGL3 and −C5118pGL3), block replacement mutations of the GATA-4 (−MμGATAUEpGL3) and NF-κB (−CμNF-κBpGL3) elements or promoterless control (pGL3) were performed. Differences between vectors are indicated by bars with unique letters (P < 0.05)

Back to article page