Fig. 6

Oxidative stress is involved in TOCP-induced autophagy of mouse Leydig TM3 cells. Mouse Leydig TM3 cells were treated with 0–200 μM H₂O₂ for 48 h. Then, cell viability was observed by MTT assay (a), and the protein levels of LC3, Atg5–Atg12, and Beclin1 were observed by Western blot; actin was used as an internal control (b). The cells were treated with 0–0.5 mM TOCP for 48 h in the absence or presence of 5 mM NAC; then cell viability (c) and the contents of LC3, Atg5–Atg12, and Beclin1 (d) were observed by MTT assay and Western blot, respectively. The experiment was done in triplicate and repeated three times. Data were analyzed by one-way ANOVA. *P < 0.05 vs. 0 mM TOCP in the absence of NAC. #P < 0.05