Fig. 3
From: Effect of nerve growth factor on sperm quality in asthenozoosprmic men during cryopreservation
Effect of nerve growth factor on sperm DNA fragmentation in asthenozoospermic men (a). Dot plot represents total acquired events and final gated (R1) population of spermatozoa (b). Histogram of negative control (without deoxynucleotidyl transferase enzyme) with 1.35 % TUNEL positive cells (M1) (c). Histogram of one of semen samples (stained by dUTP-FITC) with 9.1 % TUNEL positive cells (M2) (d). P-values <0.05 were considered significant.*: Significant difference vs. fresh group (P-value <0.05). **: Significant difference vs. frozen-thawed group (P-value <0.05). Error bars: ±1 SE