Fig. 2
From: Effect of nerve growth factor on sperm quality in asthenozoosprmic men during cryopreservation
Effect of nerve growth factor on sperm nitric oxide content in normozoospermic men (a). Dot plot represents total acquired events and final gated population (R2) of spermatozoa (b). Histogram of negative control without incubation with baseline 4, 5-diaminofluorescein-2/diacetate (DAF-2/DA) fluorescence (M1) (c). Histogram of one of semen samples, incubated with DAF-2/DA fluorescence. The population producing nitric oxide corresponds to M2 region of the histograms (d). P-values <0.05 were considered significant.*: Significant difference vs. fresh group (P-value <0.05). **: Significant difference vs. frozen-thawed group (P-value <0.05). Error bars: ±1 SE