Fig. 1
From: Effect of nerve growth factor on sperm quality in asthenozoosprmic men during cryopreservation
Effect of nerve growth factor on sperm viability in asthenozoospermic men (a). Human spermatozoa staining with Eosin/Nigrosin dye assessed by oil immersion light microscopy at × 1000 magnification. Live spermatozoa appeared white whilst dead spermatozoa with disrupted membranes have taken up the Eosin stain and appeared red (b). P-values <0.05 were considered significant.*: Significant difference vs. fresh group (P-value <0.05). **: Significant difference vs. frozen-thawed group (P-value <0.05). Error bars: ±1 SE