Table 1 List of primers used for quantitative RT-PCR amplification
Forward primer (5’-3’) | Reverse primer (5’-3’) | Product length (bp) | Annealing temperature (°C) | Gene bank accession | |
|---|---|---|---|---|---|
PTGS2 | CTG AGT ACT TTT GAC TGT GGG AG | CTC TTC CTC CTG TGC CTG AT | 359 | 60 | NM_174445 |
PLA2G4A | AAA TGT CAG CCA CAA CCC TC | ATG GAG GGT GAA AAG CG | 229 | 56 | NM_001075864.1 |
PR | GAG AGCT CAT CAA GGC AAT TGG | CAC CAT CCC TGC CAA TAT CTTG | 227 | 60 | NM_001205356.1 |
ESR1 | AGG GAA GCT CCT ATT TGC TCC | CGG TGG ATG TGG TCC TTC TCT | 234 | 58 | AY538775 |
SDHA | GGG AGG ACT TCA AGG AGA GG | CTC CTC AGT AGG AGC GGA TG | 219 | 60 | DQ386895.1 |
SUZ12 | TTC GTT GGA CAG GAG AGA CC | GTG CAC CAA GGG CAA TGT AG | 286 | 60 | NM_001205587.1 |
ACTB | CGG TGC CCA TCT ATG AGG | GAT GGT GAT GAC CTG CCC | 266 | 58 | AY141970 |
GAPDH | CCC AGA AGA CTG TGG ATG G | AGT CGC AGG AGA CAA CCT G | 306 | 32 | U85042 |