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Fig. 5 | Reproductive Biology and Endocrinology

Fig. 5

From: Analysis of 17β-estradiol (E2) role in the regulation of corpus luteum function in pregnant rats: Involvement of IGFBP5 in the E2-mediated actions

Fig. 5

Schematic representation and dendrogram generated after hierarchical clustering of differentially expressed E2 responsive genes during different treatments. a Venn diagrams representing the number of differentially expressed genes post different treatments. Data was analyzed by Bioconductor analysis tool. Thirty and 26 genes that were identified as up and down regulated after E2 inhibition that were identified as down and up regulated with E2 replacement treatment. A comparison of total number of up and down regulated genes was done across AI (orange) and AI+E2 (blue). b Heat map or combined tree of the hierarchy analysis (by clustering gene identity and conditions) for probe sets at each time point. i.e. VEH vs. AI and AI vs. AI+E2. The plot includes two dendrograms- vertical dendrogram for gene identity, and horizontal for different treatments. A visual dual colour code, red and green, is utilized to indicate relatively up or down regulation, respectively. Top of each dendrogram shows the condition colour bar with the parameters in each interpretation. c Top 40, up and down regulated genes from the analysis were utilized to construct heat map to get a view of the differentially regulated E2 regulated genes post different treatments. The top of each dendrogram shows the condition colour bar. Hovering over the cells of the heat map, the normalized intensity values are represented in various shades of red and green indicating relatively either up or down regulation, respectively. The row header shown on the right side represents the complete entity name or gene symbol. The genes Cyp19a1, Igfbp5, Igf1, Igf1r, and Esr1 are further highlighted with red arrows

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