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Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: Analysis of 17β-estradiol (E2) role in the regulation of corpus luteum function in pregnant rats: Involvement of IGFBP5 in the E2-mediated actions

Fig. 1

Effects of AI on circulating E2, luteal E2 and T, weight of CL and Cyp19a1 mRNA expression during mid pregnancy in rats. Experimental protocols from Experiment 2–5 (A-C) in Rattus norvegicus. The CL and blood collection are indicated by a red star and dot, respectively. Pregnant rats received oral administration of VEH (2 % ethanol) or AI (1 mg/kg BW) on day 7 (a) or 12 (b) for 4 days daily and blood and CL tissue samples were collected on day 11 or 16. c Beginning on day 12, pregnant rats received oral administration of AI (1 mg/kg BW) and 5 μg E2 or 4 mg/kg BW GH or 15 mg/kg BW Flu/am and pm, s.c. for 4 days daily. CL tissues collected on day 16 of pregnancy. d Mean±SEM serum E2 concentration during different treatments (n = 4 animals/time point, one-way ANOVA, *** P <0.001). Two to three corpora lutea were processed for tissue lysate preparation using sterile ice cold 1X PBS for quantitating luteal E2 (e) and T (f) concentrations. Each bar represents mean±SEM, n = 4 animals/time point, one-way ANOVA, *** P <0.001. g Weight of CL during different treatments along with a representative photo for each treatment is shown on each bar (mean±SEM, n = 12 CL/time point, one-way ANOVA, *** P <0.001). h qPCR expression of Cyp19a1 in CL post different treatments. The results are shown as fold changes in mRNA expression compared with day 12 control. Individual bars represent mean±SEM fold change in mRNA expression value for qPCR analysis during different treatments (n = 4 animals/time point, one-way ANOVA, *** P <0.001)

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