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Table 1 Oocyte development to MII arrest and 4-cell stage of embryonic development at 60 h following parthenogenetic activation

From: MicroRNA-21 and PDCD4 expression during in vitro oocyte maturation in pigs

Treatment groupc

Total oocytes maturedd

Percentage MII arrested oocytese

Percentage 4-cell or greater at 60 hf

Average blastomere # at 60 hg

Control

1646

55.4 ± 3.6a

73.0 ± 5.7a

5.8 ± 1.2 (n = 16)

NC-PNA

1136

49.0 ± 2.5a

60.2 ± 15.8ab

6.3 ± 1.4 (n = 12)

Anti-MIR21 PNA

1639

33.7 ± 3.6b

41.7 ± 12.1b

4.8 ± 1.4 (n = 12)

  1. a,bValues with different superscripts in the same column are significantly different (P < 0.05)
  2. cTreatments include: control maturation conditions, NC-PNA (2.0 nM), anti-MIR21 PNA (2.0 nM)
  3. dTotal number of GV oocytes matured for each treatment from five replications
  4. ePercentage of MII arrested oocytes from each treatment. Mean ± SEM
  5. fPercentage of embryos achieving 4-stage or greater within 60 h following parthenogenetic activation of MII arrested oocytes. Mean ± SEM
  6. gAverage number of blastomeres at 60 h based on a subset of embryos stained with nuclear stain DAPI