Fig. 4
From: MicroRNA-21 and PDCD4 expression during in vitro oocyte maturation in pigs
PDCD4 protein expression following MIR21 inhibition during IVM. PDCD4 expression abundance is presented relative to PDCD4 expression in GV oocyte samples (n = 4). From left to right: Control MII arrested oocytes, MII arrested oocytes in vitro matured in the presence of high concentration (2.0 nM) of the anti-MIR21 PNA or negative control PNA, and MII arrested oocytes in vitro matured in the presence of low concentration (0.2 nM) of the anit-MIR21 PNA or negative control PNA. Data are presented following normalization to the mean GV stage oocyte band intensity. a,bMeans ± SEM with different superscripts are different (P < 0.05). Inset is a representative Western blot of PDCD4 protein expression