Fig. 6

Oil dose–response and sensitivity of MEGA for detection of suboptimal conditions. The sensitivity of Pou5f1-GFP embryos to culture conditions was further assessed with various doses of PID oil. Pou5f1-GFP embryos cultured under oil overlays containing 0 (control), 5, 10, or 15 % PID oil were evaluated for embryo development/expression at 48 h (8-cell or EFI) and 96 h (E/H) (3 experiments). Statistical analysis: 2-way ANOVA with multiple comparisons. *p < 0.05, **p < 0.005, ****p < 0.0001. Error bars: mean ± SEM