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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: Genetic mouse embryo assay: improving performance and quality testing for assisted reproductive technology (ART) with a functional bioassay

Fig. 4

Double-blinded assessment of Pou5f1-GFP embryo development. Control and suboptimal (10 % PID oil) culture conditions were tested with micro-drop method for effect on Pou5f1-GFP embryo development and gene expression. Pou5f1-GFP embryos were evaluated at 48 h for morphology (≥8 cell) and expression (MEGA, high EFI 2–3), and subsequently assessed at 96 h for blastocyst development with localized expression of POU5F1-GFP, shown as % Embryos

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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