Table 1 Selected genes modulated by human recombinant relaxin treatment of human endometrial stromal cells in vitro
Category | Gene | Accession number | Fold-change |
|---|---|---|---|
Angiogenesis related | Tissue inhibitor of metalloproteinase-3 | NM_000362 | +1.9 |
| Â | Hypoxia-inducible factor 1, alpha subunit | NM_001530 | +1.5 |
| Â | Hepatocyte growth factor | NM_000601 | +1.5 |
| Â | Angiogenin | NM_001145 | +1.4 |
| Â | Vascular endothelial growth factor A | NM_001025366 | +1.2 |
| Â | Vascular endothelial growth factor D | NM_004469 | - 0.3 |
Angiogenesis inhibitors | CD36 | NM_001001548 | - 0.3 |
Hemostasis/menses correlated | Matrix metalloproteinase-7 | NM_002423 | - 0.4 |
| Â | Tissue plasminogen activator | NM_000930 | - 0.3 |
Secretory phase expression | Interleukin 1 receptor-like 1 | NM_003856 | +1.5 |
| Â | CD44 | NM_000610 | - 0.3 |
Proliferative phase expression | Tenascin C | NM_002160 | - 0.4 |
| Â | Androgen receptor | NM_000044 | - 0.3 |
Renin-angiotensin system | Renin | NM_000537 | +1.4 |
| Â | Angiotensin II receptor 1 | NM_000685 | +1.4 |
| Â | Angiotensin II receptor 2 | NM_000686 | - 0.3 |
Growth factors/cytokines | Insulin-like growth factor binding protein 4 | NM_001552 | +1.4 |
| Â | Insulin-like growth factor 1 | NM_001111284 | +1.4 |
| Â | Osteoclast stimulating factor 1 | NM_012383 | +1.4 |
| Â | Amphiregulin | NM_001657 | +1.4 |
| Â | Connective tissue growth factor | NM_001901 | - 0.3 |
Structural proteins | Dystrophin | NM_004011 | +1.6 |
| Â | Claudin 10 | NM_006984 | +1.5 |
| Â | Tetraspan 3 | NM_005724 | +1.4 |
| Â | Myosin X | NM_012334 | +1.4 |
| Â | Contactin 1 | NM_001843 | +1.4 |
| Â | Keratocan | NM_007035 | - 0.5 |
| Â | Matrin 3 | NM_199189 | - 0.3 |
| Â | Sorcin | NM_003130 | - 0.3 |
| Â | Cadherin-2 | NM_001792 | - 0.3 |
| Â | Tight junction protein 1 | NM_003257 | - 0.3 |
Extracellular matrix | Fibronectin | NM_212482 | - 0.3 |
| Â | Nidogen (entactin) | NM_002805 | - 0.3 |
Metabolism | Glycerol kinase | NM_203391 | +2.0 |
| Â | Acyl-Coenzyme A dehydrogenase | NM_000016 | +1.6 |
| Â | Ceruloplasm | NM_000096 | +1.4 |
| Â | Transferrin | NM_001063 | +1.4 |
Enzymes/inhibitors | Heparin cofactor II | NM_000185 | +1.8 |
| Â | Serine protease inhibitor | NM_001127698 | +1.5 |
| Â | Carboxypeptidase B2 | NM_001872 | +1.5 |
| Â | Aminoacylase 1 | NM_000666 | +1.4 |
| Â | Cathepsin B | NM_001908 | - 0.3 |
Transcription factors | Kruppel-like factor-1 | NM_006563 | +1.5 |
| Â | Eukaryotic translation termination factor 1 | NM_004730 | +1.5 |
| Â | Eukaryotic translation initiation factor 3 | NM_003758 | +1.5 |
| Â | Transactivator jun-B | NM_002229 | +1.4 |
Protein kinases | Mitogen-activated protein kinase 4 | NM_002747 | +1.5 |
| Â | Protein kinase, cAMP-dependent regulatory | NM_002734 | +1.4 |
| Â | Calcium/calmodium protein kinase I | NM_003656 | +1.4 |
| Â | Calcium/calmodium protein kinase II | NM_172127 | +1.4 |
| Â | Protein kinase, cAMP-dependent catalytic | NM_002731 | - 0.4 |
| Â | Mitogen-activated protein kinase 1 | NM_002745 | - 0.3 |
| Â | Cyclin G1 | NM_004060 | - 0.3 |
Receptors | Retinal G-protein coupled receptor | NM_002921 | +1.5 |
| Â | Hyaluronan-mediated motility receptor | NM_001142556 | +1.4 |
| Â | Vasoactive intestinal peptide receptor 2 | NM_003382 | +1.4 |
| Â | Activin A receptor IB | NM_004302 | - 0.3 |
| Â | Chemokine receptor 1 | NM_001295 | - 0.3 |
Others | Lacrimal proline-rich protein | NM_007244 | +1.6 |
| Â | Trefoil factor-1 | NM_003225 | +1.6 |
| Â | Amyloid precursor protein binding protein-2 | NM_006380 | +1.5 |
| Â | Neurogenic differentiation 1 | NM_002500 | +1.5 |
| Â | Annexin A3 | NM_005139 | +1.5 |
| Â | Basonuclin 1 | NM_001717 | - 0.5 |