Effect of bone morphogenetic protein-4 on in vitro growth, steroidogenesis and subsequent developmental competence of the oocyte-granulosa cell complex derived from bovine early antral follicles

Table 4 Effect of BMP-4 in IVG medium on the development of IVG oocytes after IVF

Oocyte	BMP-4 (ng/ml)	No. of oocytes (replicates)	% of cleaved oocytes	% of blastocysts based on		Cell no. in blastocysts (n)
Oocyte	BMP-4 (ng/ml)	No. of oocytes (replicates)	% of cleaved oocytes	Inseminated oocytes	Cleaved oocytes	Cell no. in blastocysts (n)
In vitro grown	0	257 (8)	54.9^b	9.3^b	17.0^b	91.4 ± 13.1^b (24)
In vitro grown	10	239 (7)	47.3^b	1.3^c	2.7^c	142.0 ± 45.6^d (2)
In vivo grown^e	-	150 (5)	76.7^a	25.3^a	33.0^a	164.6 ± 10.4^a (19)

^abcValues with different superscripts in the same column are significantly different (p < 0.05)
^dOne blastocyst was lost during cell counting; as a result, no statistical comparison was conducted
^eOocytes collected from antral follicles (2–8 mm in diameter) served as in vivo-derived controls

ISSN: 1477-7827