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Table 3 Effect BMP-4 in IVG medium on the fertilization of IVG oocytes

From: Effect of bone morphogenetic protein-4 on in vitro growth, steroidogenesis and subsequent developmental competence of the oocyte-granulosa cell complex derived from bovine early antral follicles

Oocyte BMP-4 (ng/ml) No. of oocytes (replicates) % of normal fertilization % of polyspermy % of total penetration
ESH 2PN subtotal
In vitro grown 0 73 (9) 16.4 43.8b 60.2b 6.8 67.0b
10 91 (9) 13.2 48.4b 61.6b 9.9 71.5b
50 53 (9) 5.7 41.5b 47.2b 7.5 54.7b
In vivo grownc - 83 (3) 6.0 80.7a 86.7a 7.2 93.9a
  1. Abbreviations: ESH enlarged sperm head, 2PN two pronuclei
  2. abValues with different superscripts in the same column are significantly different (p < 0.05)
  3. cOocytes collected from antral follicles (2–8 mm in diameter) served as in vivo-derived controls