Effect of bone morphogenetic protein-4 on in vitro growth, steroidogenesis and subsequent developmental competence of the oocyte-granulosa cell complex derived from bovine early antral follicles

Table 3 Effect BMP-4 in IVG medium on the fertilization of IVG oocytes

Oocyte	BMP-4 (ng/ml)	No. of oocytes (replicates)	% of normal fertilization			% of polyspermy	% of total penetration
Oocyte	BMP-4 (ng/ml)	No. of oocytes (replicates)	ESH	2PN	subtotal	% of polyspermy	% of total penetration
In vitro grown	0	73 (9)	16.4	43.8^b	60.2^b	6.8	67.0^b
	10	91 (9)	13.2	48.4^b	61.6^b	9.9	71.5^b
	50	53 (9)	5.7	41.5^b	47.2^b	7.5	54.7^b
In vivo grown^c	-	83 (3)	6.0	80.7^a	86.7^a	7.2	93.9^a

Abbreviations: ESH enlarged sperm head, 2PN two pronuclei
^abValues with different superscripts in the same column are significantly different (p < 0.05)
^cOocytes collected from antral follicles (2–8 mm in diameter) served as in vivo-derived controls

ISSN: 1477-7827