Fig. 6

Percentage methylated CpGs in the TIMP-1 promoter in gestational tissues cultured in the presence of lipopolysaccharide (LPS, 5 μg/ml) with/without prior treatment with 5-aza-deoxycytidine (AZA, 5 μM) (n = 8). a Villous placenta; b Amnion and c Choriodecidua. Tissues from pregnancies with male fetuses (n = 5) are shown by white bars and those from pregnancies with female fetuses (n = 3) are shown by cross-hatched bars. For graphical representation, all CpGs within region A have been combined. Representative epigrams for both male and female tissues are shown alongside each graph. * P ≤ 0.05, ** P ≤ 0.01, **** P ≤ 0.001. Percentage methylation calculated from the ratio of mass signals between methylated and non-methylated DNA in each sample. Data are presented as mean %5-mC + SEM