Fig. 5

Percentage methylated CpGs in the TIMP-1 Promoter in term villous placenta, amnion and choriodecidua collected prior to, or post labor and delivery. a Tissues collected prior to the onset of labor from elective Caesarean sections deliveries (CS, n = 14) are shown by the white boxes. Tissues collected following the spontaneous onset of labor from vaginal deliveries (SVD, n = 10) are shown by grey boxes. TIMP-1 methylation further analysed by sex of the fetus in b CS deliveries (Male n = 8, Female n = 6) and c SVD (Male n = 5 and Female n = 5). Tissues from pregnancies with male fetuses are shown by white or grey bars and those from pregnancies with female fetuses are shown by cross-hatched bars. d Representative epigrams show the % of 5-mC for each tissue at each CpG site within the amplicon for Region B. Percentage methylation calculated from the ratio of mass signals between methylated and non-methylated DNA in each sample. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Data are presented as mean %5-mC + SEM