Fig. 4

TIMP-1 protein measured by western blotting in treated explant samples shown as fold change compared to controls. Tissues were cultured in the presence of lipopolysaccharide (LPS, 5 μg/ml) with/without prior treatment with 5-aza-deoxycytidine (AZA, 5 μM; n = 8). a Villous placenta b Amnion and c Choriodecidua and further analysed by the sex of the fetus in d Villous placenta e Amnion and f Choriodecidua. Tissues from pregnancies with male fetuses (n = 5) are shown by white bars and those from pregnancies with female fetuses (n = 3) are shown by cross-hatched bars. Data are calculated as fold change compared to time matched DMSO treated control, represented by the dotted line, and presented as mean ± SEM * P ≤ 0.05. All results were normalised to beta actin optical density