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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Regulation of TIMP-1 in Human Placenta and Fetal Membranes by lipopolysaccharide and demethylating agent 5-aza-2'-deoxycytidine

Fig. 3

TIMP-1 mRNA expression as fold change compared to controls in gestational tissues measured using qRT-PCR. Tissues were cultured in the presence of lipopolysaccharide (LPS, 5 μg/ml) with/without prior treatment with 5-aza-deoxycytidine (AZA, 5 μM; n = 8). a Villous placenta b Amnion and c Choriodecidua, and further analysed by the sex of the fetus in d Villous placenta e Amnion and f Choriodecidua. Tissues from pregnancies with male fetuses (n = 5) are shown by white bars and those from pregnancies with female fetuses (n = 3) are shown by cross-hatched bars. Data are calculated as fold change compared to time matched DMSO treated control, represented by the dotted line, and presented as mean ± SEM * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001. All results were normalised to the expression of endogenous controls (RPLPO and RPL13a)

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