Fig. 4
Effect of E2 + MPA, MPA-mix (E2, MPA + cAMP) and AZA on the expression of genes known to interact with prorenin/prorenin receptor that are independent of Ang II. a PLZF (Promyelocytic leukaemic zinc finger protein) mRNA was not detected in CON or AZA treated cells but was present in E2 + MPA and MPA-mix treated cells (P < 0.001 for both treatments compared with CON and AZA). Expression of PLZF in MPA-mix treated cells was much greater than in E2 + MPA treated cells (P < 0.001). b p85α-PI3kinase (PIK3R1) mRNA expression was significantly increased in MPA-mix treated HESCs compared with those treated with E2 + MPA alone (P = 0.007) although it was not greater then control (P < 0.07). Different superscripts denote differences between groups. CON, treated with vehicle alone; E2 + MPA, treatment with 10 nM 17β-estradiol (E2) and 1 μM medroxyprogesterone acetate (MPA); E2 + MPA + cAMP, treatment as for E2 + MPA plus 0.5 mM cAMP