Fig. 2
Effects of E2 + MPA, MPA-mix and AZA on the renin angiotensin system in HESCs. a REN mRNA levels were significantly higher in HESCs treated with MPA-mix compared with all other treatments (P < 0.0001). REN mRNA expression was also increased in AZA treated HESCs compared with control and E2 + MPA treated cells (P < 0.008 and P < 0.014, respectively). b AGT mRNA levels were significantly increased in MPA-mix treated cells compared with all other treatments (all P < 0.02). c Prorenin protein (ng/ml) secreted into media at 2 days. There was no difference between CON and treatments but MPA-mix had significantly more prorenin than E2 + MPA alone (P = 0.045). d Prorenin protein (ng/ml) secreted into media at 10 days. Prorenin levels were significantly increased in the supernatant at 10 days in MPA-mix treated samples compared with all other treatments (P < 0.022). AZA treatment was associated with increased prorenin levels in supernatant compared with E2 + MPA alone (P < 0.015). Different superscripts denote differences between groups. CON, treated with vehicle alone; E2 + MPA, treatment with 10 nM 17β-estradiol (E2) and 1 μM medroxyprogesterone acetate (MPA); E2 + MPA + cAMP, treatment as for E2 + MPA plus 0.5 mM cAMP