Table 3 Expression of HSP70 in 2-cell and day 4 embryos as measured by immunofluorescence and RT-PCR
From: HSP70 overexpression may play a protective role in the mouse embryos stimulated by CUMS
Control group | Mild stress group | Moderate stress group | Severe stress group | P value | |
|---|---|---|---|---|---|
IFa | |||||
Positive 2-cell embryos | 10 %(3/30) | 87 %(26/30) | 60 %(18/30) | 13 %(4/30) | <0.05 |
Positive D4 embryos | 20 %(6/20) | 83 %(25/30) | 47 %(14/30) | 17 %(5/30) | <0.05 |
RT-PCRb | |||||
HSP70 of 2-cell embryos | 1 | 6 ± 2* | 3 ± 1* | 1.2 ± 1 | <0.05 |
HSP70 of D4 embryos | 1 | 13 ± 5* | 7 ± 3* | 1.5 ± 1 | <0.05 |