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Table 3 Expression of HSP70 in 2-cell and day 4 embryos as measured by immunofluorescence and RT-PCR

From: HSP70 overexpression may play a protective role in the mouse embryos stimulated by CUMS

  Control group Mild stress group Moderate stress group Severe stress group P value
IFa
 Positive 2-cell embryos 10 %(3/30) 87 %(26/30) 60 %(18/30) 13 %(4/30) <0.05
 Positive D4 embryos 20 %(6/20) 83 %(25/30) 47 %(14/30) 17 %(5/30) <0.05
RT-PCRb
 HSP70 of 2-cell embryos 1 6 ± 2* 3 ± 1* 1.2 ± 1 <0.05
 HSP70 of D4 embryos 1 13 ± 5* 7 ± 3* 1.5 ± 1 <0.05
  1. aData are shown as rates and were analyzed by χ 2 test and the partitioning χ 2 method
  2. bData are shown as the mean ± SD and were analyzed by ANOVA and LSD testing
  3. * P < 0.05 compared with the control group, measured by LSD test