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Table 1 Concentrationa of consistentlyb secreted cytokines in conditioned medium of isolated cytotrophoblasts exposed to AMA for 24 h

From: Cationic antimicrobial peptide, magainin down-regulates secretion of pro-inflammatory cytokines by early placental cytotrophoblasts

Cytokine (Alias)

Concentration (ng/ml) of AMA applied

0

1

10

100

1000

CCL2 (MCP1)

96.8 (3.2)

80.8 (10.2)

78.7 (14.6)

73.1** (5.2)

74.5** (5.9)

CCL3 (MIP1Α)

49.0 (2.9)

53.2 (5.2)

53.3 (5.2)

44.4 (3.4)

38.5* (2.1)

CCL4 (MIP1B)

298.0 (22.1)

298.3 (12.9)

350.6 (35.2)

265.2* (12.6)

260.2** (12.4)

CCL5 (RANTES)

362.4 (20.7)

367.9 (30.3)

362.4 (21.3)

332.0* (17.9)

309.7* (11.6)

CLEC11A (SCGFB)

592.1 (58.9)

602.9 (72.0)

609.3 (94.9)

559.7 (68.7)

544.9 (68.4)

CXCL1 (GROΑ)

678.0 (61.2)

688.5 (22.9)

649.2 (33.4)

529.4** (29.3)

527.2** (30.6)

CXCL9 (MIG)

113.2 (10.4)

112.9 (16.0)

114.2 (12.6)

97.1 (15.4)

99.4 (17.9)

CXCL10 (IP-10)

152.7 (12.5)

143.7 (14.2)

119.5 (28.0)

129.9 (29.9)

155.21 (10.74)

CXCL12 (SDF-1)

87.5 (14.1)

89.6 (26.7)

99. 9 (27.5)

83.8 (13.3)

79.6 (23.5)

FGF2 (basic FGF)

4.4 (0.3)

4.9 (0.2)

4.8 (0.2)

4.1 (0.9)

3.2* (0.5)

GCSF (CSF-3)

2307.5 (101.6)

2260.1 (138.4)

2242.3 (160.1)

2127.0 (177.3)

1946.0 (250.6)

HGF (SF)

12501.5 (1648.7)

10638.3 (1955.3)

10935.2 (1194.5)

9930.7 (874.5)

9920.1 (834.8)

IFNA2

4.9 (1.9)

11.1 (5.4)

4.5 (0.9)

4.9 (2.9)

4.7 (1.6)

IL1A

113.4 (14.9)

106.0(15.5)

112.8 (17.0)

128.7 (18.2)

120.6 (13.4)

IL1B

353.0 (23.4)

333.1 (34.2)

322.7 (31.6)

313.5* (26.9)

302.2** (10.6)

IL-1RA

32.9 (2.6)

30.2 (6.1)

27.8 (6.5)

29.5 (1.0)

28.8 (3.0)

IL-2RA (CD25)

ND

ND

2.3(i) (0.1)

2.2(i) (0.1)

2.9(i) (0.2)

IL6 (IFNÎ’2)

752.7 (41.8)

736.2 (40.1)

704.3 (31.3)

611.5** (22.6)

587.8** (26.1)

IL8 (CXCL8)

11779.0 (2146.6)

12706.4 (2868.5)

16701.7 (4785.2)

14902.2 (4615.2)

8302.9* (2435.9)

IL9 (HP40)

0.6 (0.3)

0.3 (0.2)

0.3 (0.2)

0.4 (0.2)

0.3 (0.2)

IL16 (LCF)

11.4(3.9)

11.0 (4.8)

18.7* (2.4)

18.3* (2.6)

18.8* (2.2)

IL18 (IGIF)

28.9 (2.8)

30.2 (2.3)

28.0 (3.0)

25.2 (1.7)

29.2 (1.7)

LIF (DIA)

2.8 (1.2)

2.9 (1.4)

2.9 (1.3)

2.8 (1.2)

3.1 (1.3)

MCSF (CSF1)

174.2 (16.2)

125.9* (23.6)

123.7* (21.3)

121.6* (27.9)

125.6** (7.4)

MIF (MMIF)

2919.2 (178.5)

2790.7 (211.2)

2708.6 (260.7)

2231.1* (261.3)

2154.2** (143.1)

TNF (TNF alpha)

0.6 (0.3)

0.5 (0.2)

0.4 (0.2)

0.4 (0.2)

0.4 (0.3)

VEGF-A (VPF)

0.3 (0.1)

0.2 (0.1)

0.2 (0.1)

0.2 (0.1)

0.2 (0.1)

  1. Values are shown as means with SDs in brackets. apg/mg of Bradford protein. Note that concentrations were estimated in terms of pg per unit mg of secreted protein (w/w) to avoid any bias in mass per unit volume. b ≥ 0.1 pg/mg of Bradford protein detected in at least 80 % samples. (i)induced. *P < 0.05, **P < 0.01 as compared to control. ND, values <0.1 pg/mg Bradford protein
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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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