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Table 1 Verification of selected differentially expressed genes by real time RT-PCR

From: Gene expression analysis of pig cumulus-oocyte complexes stimulated in vitro with follicle stimulating hormone or epidermal growth factor-like peptides

Probe name Gene symbol Gene description Fold change in gene expression values determined by RT-PCR (Mean ± SEM) Significance vs. non-stimulated control Array results (Fold change)
  FSH vs. Control - overexpressed    
31605:45360_15417313:f ABCC5 ATP-binding cassette, sub-family C (CFTR/MRP), member 5 0.83 ± 0.12 ns. 4.79
9436:30168_CL9Contig1:f ADAMTS1 ADAM metallopeptidase with thrombospondin type 1 motif, 1 3.72 ± 0.36 *** 6.95
13062:45360_CL23Contig1:f ANXA1 Annexun A1 0.93 ± 0.10 ns. 3.15
1117:45360_CL10Contig1:r ANXA2 Annexin A2 0.98 ± 0.07 ns. 4.02
14127:9388_21549088:r AREG Amphiregulin 5.04 ± 0.46 *** 4.06
18133:7423_CL1Contig2:r CEBPB CCAAT/enhancer binding protein (C/EBP), beta 9.68 ± 1.58 *** 2.89
6532:35657_CL1Contig1:r CYP11A1 Cytochrome P450, family 11, subfamily A, polypeptide 1 11.92 ± 0.90 *** 16.20
TC226972:f FOXO3 Forkhead box O3 1.97 ± 0.21 ** 4.22
TC235800:f HAS2 Hyaluronan synthase 2 47.63 ± 5.11 *** 6.59
9355:35407_CL1Contig1:f HSD17B7 Hydroxysteroid (17-beta) dehydrogenase 7 3.35 ± 0.30 *** 3.87
5795:24780_CL1Contig1:r MARCKSL1 MARCKS-like 1 4.44 ± 0.53 *** 2.85
1337:12192_CL1Contig2:f ODC1 Ornithine decarboxylase 1 6.22 ± 0.85 ** 5.26
15979:340_CL1Contig1:f PBX1 Pre-B-cell leukemia homeobox 1 0.70 ± 0.10 * 4.24
31453:2453_233792:f PGR Progesterone receptor 23.99 ± 5.10 ** 15.57
8538:3878_CL2Contig1:f PLAT Plasminogen activator, tissue 14.16 ± 2.36 *** 5.48
4143:1539_CL1Contig1:r PLAUR Plasminogen activator, urokinase receptor 2.56 ± 0.20 *** 4.00
33691:1766_29276590:r PRDX2 Peroxiredoxin 2 6.30 ± 0.44 *** 2.88
13439:18092_CL1Contig1:r RBKS Ribokinase 0.51 ± 0.07 *** 7.32
3373:854_CL1Contig1:f RGS2 Regulator of G-protein signaling 2 3.47 ± 0.77 * 3.13
33911:29637_CL1Contig1:r SBSN Suprabasin 20.37 ± 1.56 *** 21.54
TC204140:f SDC1 Syndecan 1 2.55 ± 0.57 * 13.44
2269:10415_CL1Contig1:f SDC4 Syndecan 4 13.79 ± 1.71 *** 6.29
TC238171:f SERPINE1 Serpin peptidase inhibitor, clade E, member 1 3.05 ± 0.50 * 14.47
31918:32605_CL1Contig2:f SMOX Spermine oxidase 33.26 ± 7.94 ** 6.29
5977:12455_CL1Contig1:r TNFAIP6 Tumor necrosis factor alpha-induced protein 6 2517 ± 105 *** 30.54
19103:6332_CL1Contig1:f TNFRSF12A Tumor necrosis factor receptor superfamily, member 12A 0.52 ± 0.17 * 3.04
9402:4028_CL1Contig1:r UAP1 UDP-N-acetylglucosamine pyrophosphorylase 1 8.01 ± 1.11 *** 5.71
  FSH vs. Control - underexpressed    
707:4250_CL1Contig1:f FOS Proto-oncogene protein c-fos 8.56 ± 2.43 * 0.15
1893:12937_CL1Contig1:f FOXO1 Forkhead box O1 0.90 ± 0.09 ns. 0.29
6206:5117_CL1Contig1:f FST Follistatin 0.21 ± 0.02 *** 0.10
15012:1275_CL1Contig1:f HS3ST1 Heparan sulfate (glucosamine) 3-O-sulfotransferase 1 0.80 ± 0.12 ns. 0.32
TC210305:f IRS1 Insulin receptor substrate 1 0.98 ± 0.25 ns. 0.14
NM_213880.1 JUN Jun proto-oncogene 1.03 ± 0.14 ns. 0.06
2480:21101_CL1Contig1:f PCNA Proliferating cell nuclear antigen 1.16 ± 0.12 ns. 0.29
18375:27730_CL1Contig1:r PNRC1 Proline-rich nuclear receptor coactivator 1 0.94 ± 0.15 ns. 0.21
14891:209_CL4Contig1:f PPARG Peroxisome proliferator-activated receptor gamma 0.45 ± 0.04 *** 0.32
1456:37319_CL1Contig1:f PTGES Prostaglandin E synthase 0.70 ± 0.10 ** 0.26
12586:12773_CL1Contig1:f RGS3 Regulator of G-protein signaling 3 0.78 ± 0.10 * 0.17
TC229705:f TRIB2 Tribbles pseudokinase 2 0.84 ± 0.10 ns. 0.33
  AREG/EREG vs. Control - overexpressed    
13062:45360_CL23Contig1:f ANXA1 Annexin A1 0.68 ± 0.11 * 3.68
1117:45360_CL10Contig1:r ANXA2 Annexin A2 2.51 ± 0.41 ** 4.41
45:916_CL3Contig1:f CTGF (CCN2) Connective tissue growth factor 2.20 ± 0.25 *** 6.19
6532:35657_CL1Contig1:r CYP11A1 Cytochrome P450, family 11, subfamily A, polypeptide 1 2.68 ± 0.44 ** 5.82
14983:2713_CL1Contig1:f EIF4E Eukaryotic translation initiation factor 4E 2.56 ± 0.72 ns. 4.14
977:13547_CL1Contig2:r ITPKA Inositol-trisphosphate 3-kinase A 5.41 ± 1.22 * 3.36
5795:24780_CL1Contig1:r MARCKSL1 MARCKS-like 1 4.96 ± 0.72 *** 3.41
31453:2453_233792:f PGR Progesterone receptor 4.01 ± 0.58 *** 5.25
33911:29637_CL1Contig1:r SBSN Suprabasin 4.33 ± 0.40 *** 4.34
TC204140:f SDC1 Syndecan 1 1.01 ± 0.01 ns. 5.88
TC238171:f SERPINE1 Serpin peptidase inhibitor, clade E, member 1 6.73 ± 0.65 *** 33.82
19103:6332_CL1Contig1:f TNFRSF12A Tumor necrosis factor receptor superfamily, member 12A 4.34 ± 0.53 ** 7.70
  AREG/EREG vs. Control - underexpressed    
707:4250_CL1Contig1:f FOS Proto-oncogene protein c-fos 3.29 ± 0.57 ** 0.09
1893:12937_CL1Contig1:f FOXO1 Forkhead box O1 1.06 ± 0.25 ns. 0.33
6206:5117_CL1Contig1:f FST Follistatin 1.01 ± 0.07 ns. 0.25
9260:5151_CL1Contig1:f GADD45A Growth arrest and DNA-damage-inducible, alpha 1.20 ± 0.32 ns. 0.35
2134:17017_CL1Contig1:f ID1 Inhibitor of DNA binding 1protein 1.24 ± 0.16 ns. 0.31
NM_213880.1 JUN Jun proto-oncogene 0.54 ± 0.02 *** 0.04
30832:5168_40390252:f NR5A2 Nuclear receptor subfamily 5, group A, member 2 1.06 ± 0.08 ns. 0.31
18375:27730_CL1Contig1:r PNRC1 Proline-rich nuclear receptor coactivator 1 0.65 ± 0.16 * 0.32
3373:854_CL1Contig1:f RGS2 Regulator of G-protein signaling 2 1.29 ± 0.09 ** 0.34
12586:12773_CL1Contig1:f RGS3 Regulator of G-protein signaling 3 0.36 ± 0.03 *** 0.13
TC229705:f TRIB2 Tribbles pseudokinase 2 0.94 ± 0.11 ns. 0.77
  1. ns. not significant. *P < 0.05; ** P < 0.01; *** P < 0.001