Table 1 Verification of selected differentially expressed genes by real time RT-PCR
Probe name | Gene symbol | Gene description | Fold change in gene expression values determined by RT-PCR (Mean ± SEM) | Significance vs. non-stimulated control | Array results (Fold change) |
|---|---|---|---|---|---|
| Â | FSH vs. Control - overexpressed | Â | Â | Â | |
31605:45360_15417313:f | ABCC5 | ATP-binding cassette, sub-family C (CFTR/MRP), member 5 | 0.83 ± 0.12 | ns. | 4.79 |
9436:30168_CL9Contig1:f | ADAMTS1 | ADAM metallopeptidase with thrombospondin type 1 motif, 1 | 3.72 ± 0.36 | *** | 6.95 |
13062:45360_CL23Contig1:f | ANXA1 | Annexun A1 | 0.93 ± 0.10 | ns. | 3.15 |
1117:45360_CL10Contig1:r | ANXA2 | Annexin A2 | 0.98 ± 0.07 | ns. | 4.02 |
14127:9388_21549088:r | AREG | Amphiregulin | 5.04 ± 0.46 | *** | 4.06 |
18133:7423_CL1Contig2:r | CEBPB | CCAAT/enhancer binding protein (C/EBP), beta | 9.68 ± 1.58 | *** | 2.89 |
6532:35657_CL1Contig1:r | CYP11A1 | Cytochrome P450, family 11, subfamily A, polypeptide 1 | 11.92 ± 0.90 | *** | 16.20 |
TC226972:f | FOXO3 | Forkhead box O3 | 1.97 ± 0.21 | ** | 4.22 |
TC235800:f | HAS2 | Hyaluronan synthase 2 | 47.63 ± 5.11 | *** | 6.59 |
9355:35407_CL1Contig1:f | HSD17B7 | Hydroxysteroid (17-beta) dehydrogenase 7 | 3.35 ± 0.30 | *** | 3.87 |
5795:24780_CL1Contig1:r | MARCKSL1 | MARCKS-like 1 | 4.44 ± 0.53 | *** | 2.85 |
1337:12192_CL1Contig2:f | ODC1 | Ornithine decarboxylase 1 | 6.22 ± 0.85 | ** | 5.26 |
15979:340_CL1Contig1:f | PBX1 | Pre-B-cell leukemia homeobox 1 | 0.70 ± 0.10 | * | 4.24 |
31453:2453_233792:f | PGR | Progesterone receptor | 23.99 ± 5.10 | ** | 15.57 |
8538:3878_CL2Contig1:f | PLAT | Plasminogen activator, tissue | 14.16 ± 2.36 | *** | 5.48 |
4143:1539_CL1Contig1:r | PLAUR | Plasminogen activator, urokinase receptor | 2.56 ± 0.20 | *** | 4.00 |
33691:1766_29276590:r | PRDX2 | Peroxiredoxin 2 | 6.30 ± 0.44 | *** | 2.88 |
13439:18092_CL1Contig1:r | RBKS | Ribokinase | 0.51 ± 0.07 | *** | 7.32 |
3373:854_CL1Contig1:f | RGS2 | Regulator of G-protein signaling 2 | 3.47 ± 0.77 | * | 3.13 |
33911:29637_CL1Contig1:r | SBSN | Suprabasin | 20.37 ± 1.56 | *** | 21.54 |
TC204140:f | SDC1 | Syndecan 1 | 2.55 ± 0.57 | * | 13.44 |
2269:10415_CL1Contig1:f | SDC4 | Syndecan 4 | 13.79 ± 1.71 | *** | 6.29 |
TC238171:f | SERPINE1 | Serpin peptidase inhibitor, clade E, member 1 | 3.05 ± 0.50 | * | 14.47 |
31918:32605_CL1Contig2:f | SMOX | Spermine oxidase | 33.26 ± 7.94 | ** | 6.29 |
5977:12455_CL1Contig1:r | TNFAIP6 | Tumor necrosis factor alpha-induced protein 6 | 2517 ± 105 | *** | 30.54 |
19103:6332_CL1Contig1:f | TNFRSF12A | Tumor necrosis factor receptor superfamily, member 12A | 0.52 ± 0.17 | * | 3.04 |
9402:4028_CL1Contig1:r | UAP1 | UDP-N-acetylglucosamine pyrophosphorylase 1 | 8.01 ± 1.11 | *** | 5.71 |
| Â | FSH vs. Control - underexpressed | Â | Â | Â | |
707:4250_CL1Contig1:f | FOS | Proto-oncogene protein c-fos | 8.56 ± 2.43 | * | 0.15 |
1893:12937_CL1Contig1:f | FOXO1 | Forkhead box O1 | 0.90 ± 0.09 | ns. | 0.29 |
6206:5117_CL1Contig1:f | FST | Follistatin | 0.21 ± 0.02 | *** | 0.10 |
15012:1275_CL1Contig1:f | HS3ST1 | Heparan sulfate (glucosamine) 3-O-sulfotransferase 1 | 0.80 ± 0.12 | ns. | 0.32 |
TC210305:f | IRS1 | Insulin receptor substrate 1 | 0.98 ± 0.25 | ns. | 0.14 |
NM_213880.1 | JUN | Jun proto-oncogene | 1.03 ± 0.14 | ns. | 0.06 |
2480:21101_CL1Contig1:f | PCNA | Proliferating cell nuclear antigen | 1.16 ± 0.12 | ns. | 0.29 |
18375:27730_CL1Contig1:r | PNRC1 | Proline-rich nuclear receptor coactivator 1 | 0.94 ± 0.15 | ns. | 0.21 |
14891:209_CL4Contig1:f | PPARG | Peroxisome proliferator-activated receptor gamma | 0.45 ± 0.04 | *** | 0.32 |
1456:37319_CL1Contig1:f | PTGES | Prostaglandin E synthase | 0.70 ± 0.10 | ** | 0.26 |
12586:12773_CL1Contig1:f | RGS3 | Regulator of G-protein signaling 3 | 0.78 ± 0.10 | * | 0.17 |
TC229705:f | TRIB2 | Tribbles pseudokinase 2 | 0.84 ± 0.10 | ns. | 0.33 |
| Â | AREG/EREG vs. Control - overexpressed | Â | Â | Â | |
13062:45360_CL23Contig1:f | ANXA1 | Annexin A1 | 0.68 ± 0.11 | * | 3.68 |
1117:45360_CL10Contig1:r | ANXA2 | Annexin A2 | 2.51 ± 0.41 | ** | 4.41 |
45:916_CL3Contig1:f | CTGF (CCN2) | Connective tissue growth factor | 2.20 ± 0.25 | *** | 6.19 |
6532:35657_CL1Contig1:r | CYP11A1 | Cytochrome P450, family 11, subfamily A, polypeptide 1 | 2.68 ± 0.44 | ** | 5.82 |
14983:2713_CL1Contig1:f | EIF4E | Eukaryotic translation initiation factor 4E | 2.56 ± 0.72 | ns. | 4.14 |
977:13547_CL1Contig2:r | ITPKA | Inositol-trisphosphate 3-kinase A | 5.41 ± 1.22 | * | 3.36 |
5795:24780_CL1Contig1:r | MARCKSL1 | MARCKS-like 1 | 4.96 ± 0.72 | *** | 3.41 |
31453:2453_233792:f | PGR | Progesterone receptor | 4.01 ± 0.58 | *** | 5.25 |
33911:29637_CL1Contig1:r | SBSN | Suprabasin | 4.33 ± 0.40 | *** | 4.34 |
TC204140:f | SDC1 | Syndecan 1 | 1.01 ± 0.01 | ns. | 5.88 |
TC238171:f | SERPINE1 | Serpin peptidase inhibitor, clade E, member 1 | 6.73 ± 0.65 | *** | 33.82 |
19103:6332_CL1Contig1:f | TNFRSF12A | Tumor necrosis factor receptor superfamily, member 12A | 4.34 ± 0.53 | ** | 7.70 |
| Â | AREG/EREG vs. Control - underexpressed | Â | Â | Â | |
707:4250_CL1Contig1:f | FOS | Proto-oncogene protein c-fos | 3.29 ± 0.57 | ** | 0.09 |
1893:12937_CL1Contig1:f | FOXO1 | Forkhead box O1 | 1.06 ± 0.25 | ns. | 0.33 |
6206:5117_CL1Contig1:f | FST | Follistatin | 1.01 ± 0.07 | ns. | 0.25 |
9260:5151_CL1Contig1:f | GADD45A | Growth arrest and DNA-damage-inducible, alpha | 1.20 ± 0.32 | ns. | 0.35 |
2134:17017_CL1Contig1:f | ID1 | Inhibitor of DNA binding 1protein | 1.24 ± 0.16 | ns. | 0.31 |
NM_213880.1 | JUN | Jun proto-oncogene | 0.54 ± 0.02 | *** | 0.04 |
30832:5168_40390252:f | NR5A2 | Nuclear receptor subfamily 5, group A, member 2 | 1.06 ± 0.08 | ns. | 0.31 |
18375:27730_CL1Contig1:r | PNRC1 | Proline-rich nuclear receptor coactivator 1 | 0.65 ± 0.16 | * | 0.32 |
3373:854_CL1Contig1:f | RGS2 | Regulator of G-protein signaling 2 | 1.29 ± 0.09 | ** | 0.34 |
12586:12773_CL1Contig1:f | RGS3 | Regulator of G-protein signaling 3 | 0.36 ± 0.03 | *** | 0.13 |
TC229705:f | TRIB2 | Tribbles pseudokinase 2 | 0.94 ± 0.11 | ns. | 0.77 |