The association of serum estradiol level with outcomes of clomiphene citrate/human menopausal gonadotropin ovarian stimulation for in vitro fertilization and embryo transfer

Table 2 Serum E₂ levels and ovarian stimulation and fertilization outcomes

	Group A:	Group B:	Group C:	P	P _adj ^e
	E₂ ≤30 pg/ml	30< E₂ ≤50 pg/ml	E₂ >50 pg/ml	P	P _adj ^e
Number of cycles	316	451	313
FSH during ovulation stimulation, IU/L^a	9.5 (7.5, 11.8)	8.9 (7.5, 10.6)	9 (7.7, 11)	0.098	0.047
Duration of stimulation, days^a	9 (8, 10)	8 (7, 10)*	8 (7, 9)* **	<0.001	<0.001
Gonadotropin, ampules	16 (12.5, 18)	14 (10, 18)*	14 (10.8, 17.5)*	<0.001	<0.001
E₂ at HCG day, pg/ml^a	2216 (1391, 3319)	2150.5 (1406, 3294)	2179.5 (1337.5, 3223)	0.991	0.976
Number of eggs^a	6 (3, 9)	5 (3, 8)	5 (2, 7)*	0.004	0.031
Number of mature oocytes (MII)^a	5 (3, 8)	4 (2, 7)	4 (2, 6)*	0.003	0.032
Number of embryos^a	2 (1, 4)	2 (1, 4)	2 (1, 4)	0.340	0.771
Number of good quality embryos^a	2 (1, 4)	2 (1, 3)	2 (1, 3)	0.440	0.638
Successful fertilization^b,c	1470 (82.7)	1968 (84.2)	1261 (82.9)	0.375	0.756
Successful cleavage^b,d	1432 (97.4)	1917 (97.4)	1217 (96.5)	0.257	0.995
Cancelled cycles^b	46 (14.6)	55 (12.2)	52 (16.6)	0.221	0.290 (Group B), 0.766 (Group C)

E ₂ estradiol, HCG human chorionic gonadotropin
Continuous data are presented as median (interquartile range), and examined by Kruskal-Wallis^a test. Categorical data are expressed as n (%) and tested by Chi-square^b test
^cFertilization rate was calculated as: total number of fertilized oocytes/total number of mature oocytes
^dCleavage rate was calculated as: total number of cleaved oocytes/total number of fertilized oocytes
^eRank transformation was applied to continuous data, and tested by analysis of covariance adjusting for age, body mass index (BMI), and baseline E₂ level. Successful fertilization and cleavage rate were tested by Poisson regression adjusting for age, BMI, and baseline E₂ level. Probability of cancelled cycle was examined by logistic regression adjusting for age, BMI, and baseline E₂ level
*Indicates significant difference between group A and given group, P < 0.017
*Indicates significant difference between group B and group C, P < 0.017

ISSN: 1477-7827