Table 1 Oligonucleotide sequences used for real-time PCR
From: Expression of pluripotency markers in the bovine uterus with adenomyosis
Gene | Oligonucleotide sequences | Product size | GeneBank |
|---|---|---|---|
(bp) | |||
vimentin | FWD 5’-GACCTGGAGCGTAAAGTGG-3’ | 108 | BC118269 |
REV 5’-GACATGCTGTTCTTGAATCTGG-3 | |||
desmin | FWD 5’-GACCCAGGCAGCCAACAAG-3’ | 100 | BC133410 |
REV 5’-GTCGATCTCGCAGGTGTAGG-3’ | |||
NANOG | FWD 5’-TGCATTTGCTGGAGACTGAG-3’ | 107 | DQ069776 |
REV 5’- GTCCCGGTCAAGAAACAAAA-3’ | |||
OCT4 | FWD 5’-AGGTGTTCAGCCAAACGACTA-3’ | 195 | FD381287.1 |
REV 5’-TCTCCTGCAGATTCTCGTTGT-3’ | |||
SOX2 | FWD 5’-GCACATGAACGGCTGGAGCAACG-3’ | 218 | JQ231229.1 |
REV 5’-TGCTGCGAGTAGGACATGCTGTAGG-3’ | |||
GAPDH | FWD 5’-CACCCTCAAGATTGTCAGCA-3’ | 103 | BC102589 |
REV 5’-GGTCATAAGTCCCTCCACGA-3’ |