Fig. 5

Immunodetection of pluripotency markers in uterine tissues from control cows and from cows with adenomyosis. a, b – haematoxylin and eosin stained slides of control and adenomyotic uterus, respectively; c, d – NANOG immunodetection in control and adenomyotic tissue, respectively; e, f – immunolocalisation of OCT4 in normal and adenomyotic tissue, respectively; g, h – SOX2 immunodetection in control and adenomyotic tissue, respectively; i, j, k – no Ab, negative controls for NANOG, OCT4 and SOX2, respectively. Unspecific IgG controls (pictures not shown) served similar pictures as no Ab control. Arrows indicate the most intense histochemical reactions; dotted line indicate endometrial-myometrial border; e – endometrium, m – myometrium, es – endometrial stroma, eg – endometrial gland, a – adenomyotic lesion, v – vessel. Scale bars: 20 μm