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Fig. 5 | Reproductive Biology and Endocrinology

Fig. 5

From: Human sperm degradation of zona pellucida proteins contributes to fertilization

Fig. 5

Effects of protease inhibitors on sperm function. (a) Effects on motility: Percentages of progressive motility (dark grey bars), non-progressive motility (white bars) and immotility (light grey bars) after sperm incubation in the presence of MG-132, O-phenanthroline and benzamidine. (b) Effects on acrosome reaction: Percentage of spontaneous (grey bars) and calcium ionophore (CaI) induced (black bars) acrosome reaction of sperm treated with MG-132, O-phenanthroline and benzamidine. Acrosomal status was evaluated after staining with FITC-PSA and percentage of reacted cells were determined. Values in percentage are presented as the mean ± SEM. No significant differences between controls (in the absence of inhibitors) and treatments were detected. N ≥ 3 experiments

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