Fig. 4From: Human sperm degradation of zona pellucida proteins contributes to fertilizationSperm degradation of rhZP proteins. Agarose beads immobilized rhZP2, rhZP3 and rhZP4 proteins were co-incubated under capacitating conditions with human spermatozoa for 16 h. Afterwards, beads were washed and bound proteins were solubilized in Laemmli denaturing sample buffer for Western blot analysis with anti-HSPZ. Control incubations without rhZP proteins and without sperm are also presented. Black arrows indicate the expected full length for each rhZP protein. Spm = spermatozoaBack to article page