Fig. 1
From: Human sperm degradation of zona pellucida proteins contributes to fertilization
Presence of ubiquitin in human ovary tissue. Immunofluorescent analysis of formalin fixed ovary sections, using for primary detection either anti zona pellucida serum (anti-HZPZ) (a), total gamma globulins from rabbit (b) or anti-Ub antibodies (c and d). Afterwards, all slices where incubated with secondary antibody and mounted for examination using phase-contrast (left panels) or epifluorescence (right panels). Ub was mostly detected inside oocytes and no signal was detected in the ZP (indicated with white arrows) of oocytes from early or developed follicles. Original magnification x400