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Fig. 7 | Reproductive Biology and Endocrinology

Fig. 7

From: Interaction of milk proteins and Binder of Sperm (BSP) proteins from boar, stallion and ram semen

Fig. 7

Gel filtration analysis of the interaction between gelatin-adsorbed ram BSP proteins and milk fraction F1. a Chromatogram of gelatin-adsorbed ram BSP proteins (500 μg) incubated with milk fraction F1 (4.8 mg). Tubes were pooled to provide seven fractions (I, tubes 42–52; II, tubes 53–70; III, tubes 71–90; IV, tubes 91–100; V, tubes 101–110; VI, tubes 111–120 and VII, tubes 121–150). b Immunoblot analysis of chromatographic fractions. SP: ram SP proteins (300 ng). Aliquots equivalent to 5 % of the fractions were precipitated. Duplicates were carried out and the results of one typical experiment are shown

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