Fig. 4

Gel filtration analysis of the interaction between pure boar BSP1 proteins and isolated milk fractions F1 and F2. a, b Chromatogram of purified boar BSP1 (100 μg) incubated with milk fraction F1 (2.9 mg) or milk fraction 2 (16.2 mg) respectively. Following the elution from both columns, tubes were pooled to provide seven fractions (I, tubes 41–47; II, tubes 48–67; III, tubes 68–87; IV, tubes 88–95; V, tubes 96–104; VI, tubes 105–113 and VII, tubes 114–146). c, d Immunoblot analysis of chromatographic fractions. Aliquots equivalent to 20 % of the pooled fractions were precipitated for the analysis. Duplicates were carried out and the results of one typical experiment are shown