Fig. 2
From: Interaction of milk proteins and Binder of Sperm (BSP) proteins from boar, stallion and ram semen
Isolation and identification of ram BSP proteins. a Chromatogram of ram SP proteins (150 mg) on gelatin-agarose column. The gelatin-adsorbed (GA) proteins (tubes 55–61) were pooled, desalted and freeze-dried. b SDS-PAGE of ram SP proteins (20 μg) and of GA fraction (15 μg). c Immunoblot analysis of ram BSP proteins using the polyclonal antibodies directed against ram BSP proteins. Ram SP proteins (2 μg) and GA proteins (200 ng). d Specificity of the polyclonal antibodies directed against ram BSP proteins. SP, ram seminal plasma proteins (300 ng) and SM, skimmed milk proteins (10 μg)