Fig. 1
From: Interaction of milk proteins and Binder of Sperm (BSP) proteins from boar, stallion and ram semen
Isolation and identification of stallion BSP proteins. a Chromatogram of stallion SP proteins (150 mg) on gelatin-agarose column. The gelatin-adsorbed (GA) proteins (tubes 55–75) were pooled, desalted and freeze-dried. b SDS-PAGE pattern of the stallion SP proteins (15 μg) and of the GA fraction (10 μg). c Immunoblot analysis of stallion BSP proteins using the polyclonal antibodies directed against stallion BSP proteins. SP proteins (2 μg) and GA proteins (200 ng). d Specificity of the polyclonal antibodies directed against stallion BSP proteins. SP, stallion seminal plasma proteins (300 ng) and SM, skimmed milk proteins (10 μg). e LC-MS/MS analysis of the GA proteins. Bold letters represent residues identified by LC-MS/MS