Fig. 3

The MMP-9 expression levels and enzymatic activities were detected with western blots and gelatin zymography, respectively, after various treatments. a Typical bands from western blots and the histogram are shown for OPN-specific siRNA treatments. 1, 3 and 5 are siRNA-scrambled; 2, 4 and 6 are siRNA-OPN. b Representative bands of gelatin zymography and the histogram show the enzymatic activities of MMP-9. The histogram represents three independent assays. Data are presented as the mean ± S.E. *, p < 0.05 for differences between cell types with the same treatment